The polymerase chain reaction (PCR) is a biochemical technology in molecular diagnostics that amplifies a single copy or a few strands of DNA across several orders of magnitude, generating several hundred thousands of copies of a particular DNA sequence. The method relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA. PCR requires a high number of thermal cycle steps to create thousands of strands of DNA sequencing for analysis. This is a harsh environment for standard thermoelectric modules due to the mechanical stresses that occur during heating and cooling stage. Recent advances in thermoelectric module (TEM) technology have improved thermal cycling efficiency, reliability and operating life to lower the overall cost of ownership of PCR equipment.