The PCR market is evolving towards faster chemistries. These advancements will speed the detection of pathogens and treatment solutions. The PCR evaluation kit demonstrates the capabilities of dramatically speeding the temperature ramps over current technologies thus reducing detection and solution times. The evaluation kit includes a thermoelectric assembly attached to a mounting stage with a controlled heat rejection side temperature. The heat rejection side is intended to maintain a constant temperature to facilitate predictable temperature performance. The cycler and mounting stage assembly are tethered to a programmable controller. The controller is then tied to a computer running an application provided to set up and monitor temperature profiles.